Figure 3.

Migration quantification in control collagen (a–d) and fibrin (e–h) gels. Polar histograms (a, e) show the directionality of cell migration and represent the angle formed by the Euclidian distance between the initial and last points of every cell trajectory, being 90° the direction of the settled gradient. The histogram bins correspond to 36° and their radius magnitude represents the number of cells (radial number) that ended within that angular range. The gradient direction is illustrated by the black arrow, whose origin corresponds to the gradient source. The trajectories of individual cells are outlined (b, f); colors indicate the zone of the microdevice in which cells were located in the last time step (red corresponding to zone 1, green to zone 2 and blue to zone 3, respectively). Boxplots show the mean and effective speed of cells considering the whole device (c, g) or distinguishing the zone they belong to (d, h). Additional statistical data corresponding to polar histograms and boxplots are shown in the Supplementary Data.