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. 2015 Apr 14;16(6):898–911. doi: 10.1080/15384047.2015.1028702

Figure 5.

Figure 5.

Overexpression of ELMO1 in H4 cell enhanced cell invasion and mesenchymal properties. (A) Overexpression of ELMO1 protein in H4 cells was detected by Western blot. β-actin was used as a loading control. (B) Comparison of chemotactic responses with IL-8 (0, 10, 100, 500, 1000 ng/ml) stimulation in control cells(H4/Con) and ELMO1 overexpression cells (H4/ELMO1). Columns, mean of triplicate measurements; Bars, standard deviation. *P < 0.05 (2-way ANOVA). (C) Comparison of invasive ability of H4/Con and H4/ELMO1 cells in culture medium of CM or PM. Columns, mean of triplicate measurements; Bars, standard deviation. *P < 0.05 (2-way ANOVA). (D) Time course of relative F-actin content in H4/Con and H4/ELMO1 cells following 10 ng/ml of IL-8 stimulation. Data from 3 independent experiments (n = 3). (E) Expression of active RAC1 protein in H4/Con and H4/ELMO1 cells with culture medium of CM or PM was detected by Western blot. RAC1 was used as a loading control. Each result is representative from at least 3 independent experiments. Quantification of relative protein levels is shown below the blots. (F) Expression of N-cadherin in H4/Con and H4/ELMO1 cells cultured in CM at different time points was detected by Western blot. β-actin was used as a loading control. Quantification of relative protein levels is shown below the blots. Each result is representative from at least 3 independent experiments.