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. 2014 Nov 20;15(12):1658–1666. doi: 10.4161/15384047.2014.972183

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© 2014 Taylor & Francis Group, LLC

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Figure 3. — Apoptosis analyses of pancreatic carcinoma cells treated with rTRAIL^wt and rTRAIL^DR4.(A) Colo357 cells were treated with PBS (gray) as control, 100 ng/ml rTRAIL^wt (rwt; brown) and rTRAIL^DR4 (rDR4; green) for 24 h before apoptosis was measured. The apoptosis was significantly higher with rTRAIL^DR4 than rTRAIL^wt. (B) PancTu1.shctrl (filled) and PancTu1.shXIAP (dashed) cells were treated with PBS (gray) as control, 100 ng/ml rTRAIL^wt (rwt; brown) and rTRAIL^DR4 (rDR4; green) for 24 h before apoptosis was measured. The results show that PancTu1.shctrl cells are TRAIL resistant and that silencing of XIAP (in PancTu1.shXIAP) sensitizes the cells. In PancTu1.shXIAP rTRAIL^DR4 induces significantly more apoptosis than rTRAIL^wt. In the insert the knock-down of XIAP is shown by Western blot of protein lysates from PancTu1.shXIAP (lane 1) and PancTu1.shctrl (lane 2) cells. The CuZnSOD blot serves as loading control.