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. 2015 Jun 28;61(5):431–437. doi: 10.1262/jrd.2014-163

Fig. 1.

Fig. 1.

Cell numbers in blastocysts derived from fresh bovine IVM oocytes (fresh control), bovine IVM oocytes treated with vitrification solution (solution control) or bovine IVM oocytes vitrified by the MVAC or Cryotop methods after IVF and in vitro culture for 9 days. Data presented as the mean no. of nuclei ± SEM. Fresh control: IVM oocytes without any vitrification treatments. Solution control: IVM oocytes that were exposed to vitrification and warming solutions. MVAC: IVM oocytes that were vitrified by inserting the MVAC device containing them into a precooled 0.25-ml plastic straw. MVAC in LN2: IVM oocytes that were vitrified by plunging the MVAC device containing them directly into LN2. Cryotop: IVM oocytes that were vitrified by the Cryotop method. ICM: inner cell mass. TE: trophectoderm. No significant differences in ICM and TE cell numbers were detected between the treatment groups at P < 0.05 using one-way ANOVA.