. 2015 Jun 28;61(5):431–437. doi: 10.1262/jrd.2014-163

Table 1. In vitro development of fresh bovine IVM oocytes (fresh control), bovine IVM oocytes treated with vitrification solution (solution control) and bovine IVM oocytes vitrified using the MVAC or Cryotop device after IVF and in vitro culture for 9 days.

Treatment group	No. of IVF oocytes	Oocytes cleaved and developed into blastocysts (%)
		Cleaved (day 2*)		Blastocyst (BL)
		Total	≥ 5 Cells	Day 7	Day 8	Day 9	Total BL
Fresh control	150	134	90	48	14	3	65
Fresh control		(89.3)^a	(60.0)^a	(32.0) ^a	(9.3)^a	(2.0)^a	(43.3)^a
Solution control	150	131	93	43	15	5	63
Solution control		(87.3)^a	(62.0)^a	(28.7)^a	(10.0)^a	(3.3)^a	(42.0)^a
MVAC	145	77	59	26	6	1	33
MVAC		(53.1)^b	(40.7)^b	(17.9)^b	(4.1)^b	(0.7)^b	(22.9)^b
MVAC in LN₂	145	81	60	24	4	1	29
MVAC in LN₂		(55.9)^b	(41.4)^b	(16.6)^b	(2.8)^b	(0.7)^b	(20.0)^b
Cryotop	145	82	63	30	5	2	37
Cryotop		(56.6)^b	(43.5)^b	(20.7)^b	(3.5)^b	(1.4)^b	(25.5)^b

Five replications were performed. ^a^,^b Values within a single column that have different superscripts are significantly different at P < 0.05 using one-way ANOVA. * Day 0 was defined as the day IVF was performed. MVAC: IVM oocytes that were vitrified by inserting the MVAC device containing them into a precooled 0.25-ml plastic straw. MVAC in LN₂: IVM oocytes that were vitrified by plunging the MVAC device containing them directly into LN₂. Cryotop: IVM oocytes that were vitrified by the Cryotop method.