. 2015 Jun 28;61(5):431–437. doi: 10.1262/jrd.2014-163

Table 2. Development to the hatched blastocyst stage of in vitro-produced expanded blastocysts vitrified using the MVAC or Cryotop device, warmed and cultured in vitro for 72 h.

Vitrification method	No. of cryopreserved embryos	No. of hatched embryos (%)
Vitrification method	No. of cryopreserved embryos	24 h	48 h	72 h
Solution control	117	73^a	94	109
Solution control	117	(62.4%)	(80.3%)	(93.2%)
MVAC	110	53^ab	88	102
MVAC	110	(48.2%)	(80.0%)	(92.7%)
MVAC in LN₂	114	40^b	82	104
MVAC in LN₂	114	(35.1%)	(71.9%)	(91.2%)
Cryotop	114	61^ab	89	102
Cryotop	114	(53.5%)	(78.1%)	(89.5%)

Six replications were performed. ^{a, b} Values within a single column that have different superscripts are significantly different at P < 0.05 using one-way ANOVA. Solution control: IVP expanded blastocysts that were exposed to vitrification and warming solutions. MVAC: IVP expanded blastocysts that were vitrified by inserting the MVAC device containing them into a precooled 0.25-ml plastic straw. MVAC in LN₂: IVP expanded blastocysts that were vitrified by plunging the MVAC device containing them directly into LN₂. Cryotop: IVP expanded blastocysts that were vitrified by the Cryotop method. No significant difference in development to the hatched blastocyst stage was detected between the treatment groups at P < 0.05 using one-way ANOVA.