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Generation of homozygous CMAH KO cloned pigs. (A) A TALEN-induced homozygous CMAH KO male piglet, M129-1, at 20 days of age. (B) DNA sequence analysis of CMAH in the cloned pigs, M129-1, 2, 3 and 4. The sequences of M129-3 (female) and M129-1, 2 and 4 (male) showed the same mutation as the sequences of the nuclear donor cells, #85 and #151, respectively. The deletion mutation is indicated by an asterisk.
