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. 2015 Jul 27;61(5):473–484. doi: 10.1262/jrd.2015-059

Fig. 1.

Fig. 1.

Development of PSC colonies by double KD of Dmrt1 and Trp53. (A) NANOG expression in PSC colonies that developed from PGCs of different stages. (B) Development of PSC colonies from GSCs by double KD of Dmrt1 and Trp53. (C) Frequency of PSC colony development after KD of the indicated genes in PGCs from 12.5 dpc embryos (n = 10). (D) Lack of PSC colony development from 15.5 and 18.5 dpc embryos and CD9-selected SSCs from 8–10 dpp pups. TRA98 immunostaining was used to detect surviving germ cells. (E) NANOG expression in a PSC colony developed by transfections of Yamanaka factors into CD9-selected SSCs from 8–10 dpp pups. Bar = 200 μm (A, B, D, E).