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. 2015 Jul 27;61(5):473–484. doi: 10.1262/jrd.2015-059

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©2015 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 5. — Production of chimeric animals. (A) Abnormal embryo development after PGC-PSC injection. Arrows and asterisks indicate placenta-only conceptuses with or without a degenerating fetus, respectively. Arrowheads indicate deciduae, which were found at the implantation sites. (B) Chimeric offspring born after microinjection of the indicated cell types into blastocysts. The white coat color represents the contribution of the donor cells (ICR background). RFP fluorescence also shows the contribution of GSC-PSCs to the testes of chimeric offspring. (C) Bisulfite sequencing analysis of H19, Igf2r and IG-DMR in PGC-PSCs. (D) Bisulfite sequencing analysis of the IG-DMR in GSC-PSCs. (E) Summary of the experiment. Bar = 250 μm (B).