Typical single-channel recordings and gating models. (A) Model topologies used in QuB to idealize single-channel recordings. Model M1 (i) comprises six states (full open, closed, and four subconductance open states S1–S4 (12)), with all-to-all connectivity between states. Model M2 (ii) is a simplified subconductance model consisting of the full open and closed states and a single, merged subconductance state encompassing a noisy combination of all substates S1–S4. (B) Representative single-channel recordings illustrating typical opening bursts at +30 and −30 mV. (C) Expanded traces show typical subconductance state open events. The red lines show the average current level of the merged subconductance state. To see this figure in color, go online.