Quiescence during DTQ and SIQ is mediated by different neuropeptides. A, Schematic representing the known pathways that control quiescence during DTQ and SIQ. In DTQ, the RIA neurons release NLP-22, whereas the RIS neuron releases an unidentified peptide. The aptf-1 gene is required for the function of the RIS neuron and the ceh-17 gene is required for the function of the ALA neuron. During SIQ, the ALA neuron releases the FLP-13 neuropeptides. It is unknown how these neuropeptides lead to behavioral quiescence. B, Feeding quiescence during SIQ does not require pathways that regulate DTQ. Mutants defective in RIA and RIS signaling (nlp-22 and aptf-1 mutants, respectively) have normal feeding quiescence during SIQ. Each point represents an observation from one worm and the horizontal bar represents the median of each group. n = 15 for each group. Statistical significance was calculated using the Mann–Whitney test. *p < 0.05.