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. 2015 Mar 17;16(3):420–429. doi: 10.1080/15384047.2014.1003003

Figure 2.

Figure 2.

Neddylation inhibition with MLN4924 triggers G2 cell-cycle arrest and suppresses the growth of lymphoma cells. (A) MLN4924 induced G2-M cell-cycle arrest. Raji, U937, SU-DHL-4 and Toledo cells seeded into 6-well plates were treated with MLN4924 at 0.1 and 0.3 μM or DMSO for 48 h, followed by PI staining and FACS analysis for cell-cycle profile. (B) MLN4924 induced accumulation of Wee1 and decrease of p-H3. The four lymphoma cells were treated with MLN4924 at 0.1 and 0.3 μM or DMSO for 48 h, and subjected to immunoblotting using antibodies against Wee1 and p-H3 (S10) with GAPDH as a loading control. (C) MLN4924 inhibited the proliferation of lymphoma cells. The four cells seeded into 24-well plates were treated with 0.3 μM MLN4924 or DMSO for indicated time points, followed by cell counting (n = 3). (D) MLN4924 impaired cell viability in lymphoma cells. The four cells seeded into 96-well black plates were treated with MLN4924 at 0.1 and 0.3 μM or DMSO for 96 h, followed by cell viability analysis using the ATPlite assay (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.