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. 2015 Jun 3;10(5):e1000103. doi: 10.1080/15592324.2014.1000103

Figure 2.

Figure 2.

RAN1 and NUP62 expression in Nicotiana benthamiana leaves. RNA and protein was extracted from N.benthamiana leaves 2 days after inoculation with only agrobacteria or with agrobacteria containing an empty pEAQ-DEST3 plasmid, pEAQ-RAN1 plasmid or pEAQ-AtNUP62 plasmid (A) RT-PCR showing expression of AtRAN1-His, AtNUP62-His or endogenous NbRAN1. NbRAN1 was used as an internal control showing no genomic DNA contamination. (B) Western blot showing expression of 6xHis-tagged AtRAN1. NS- Non-specific band visualised by Ponceau Red staining. Sample numbers: 1- No plasmid 2- pEAQ-AtRAN1 3- pEAQ-DEST3. 1a- No plasmid 2a- pEAQ-AtNUP62 3a- pEAQ-DEST3. cDNA +ve/-ve represents presence of RT enzyme in cDNA preparation mix.