Figure 6.

Enhancement of 5-FU-mediated anti-HT-29 cell activity by INK-128. HT-29 cells were treated with INK-128 (5 nM), or with 5-FU (5 μM) for 72 h, cell viability and cell death were tested by MTT assay (A) and PI staining assay (B), respectively. Mice bearing HT-29 tumors were administrated 3 weeks with: vehicle (“Veh”), INK-128 (1 mg/kg, p.o. daily), 5-FU (30 mg/kg, i.p. twice a week), MEK-162 (2.5 mg/kg, p.o. daily), INK-128 + 5-FU, or INK-128 + MEK-162, n = 8 per group. Mean Tumor Volume (in mm³, recorded every week) and average tumor growth (in mm³/week) were presented (C and D). Data were expressed as mean ± SD, experiments were repeated 3 times, and similar results were obtained. *P < 0 .05 vs. vehicle or “C” group. **P < 0 .05 vs. Five-FU only group.