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. 2014 Dec 17;9(12):e977746. doi: 10.4161/15592324.2014.977746

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Figure 1. — Analysis of peroxidase (POD) activity in total soluble protein (10,000g supernatant) from dark-grown sunflower seedling cotyledons grown in the absence or presence of 120 mM NaCl. (A) Spectrophotometric determination of specific activity of POD. NaCl^-stress induced changes in enzyme activity were analyzed by one-way ANOVA using SPSS 22.0 and were found to be statistically significant (***P < 0.001) in comparison to control [(-)NaCl]. Bars represent SE (n = 3). (B) Zymographic detection of POD isoforms. (C) Western blot analysis of POD using anti-peroxidase antibody.