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. 2014 Dec 17;9(12):e977746. doi: 10.4161/15592324.2014.977746

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Figure 2. — Analysis of phospholipid hydroperoxide glutathione peroxidase (PHGPX) activity in total soluble protein (10,000g supernatant) from dark-grown sunflower seedling cotyledons grown in the absence or presence of 120 mM NaCl. (A) Quantification of lipid hydroperoxide content using PeroxiDetect Kit (Sigma-Aldrich Chemicals Pvt. Ltd., USA). Changes in lipid hydroperoxide content were found to be statistically significant (**P < 0.01, ***P < 0.001), as analyzed by one-way ANOVA using SPSS 22.0. Bars represent SE (n = 3). (B) Spectrophotometric determination of specific activity of PHGPX. NaCl^-stress induced changes in enzyme activity, were analyzed by one-way ANOVA using SPSS 22.0 and were found to be statistically significant (*P < 0.05, **P < 0.01, ***P < 0.001) in comparison to control [(-)NaCl]. Bars represent SE (n = 3). (C) Western blot analysis of PHGPX using anti-GPX4 (PHGPX) antibody.