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. 2014 Dec 17;9(12):e977746. doi: 10.4161/15592324.2014.977746

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Figure 3. — Localization of phospholipid hydroperoxide glutathione peroxidase (PHGPX) by CLSM imaging using anti-GPX4 (PHGPX) antibody. (A) Visible image of 7 μm thick transverse sections of cotyledon from 2 d-old etiolated seedlings. Fluorescence micrographs of cotyledon transverse sections in the absence of anti-GPX4 (PHGPX) antibody (B), in the presence of anti-GPX4 (PHGPX) antibody in cotyledons from (−) NaCl seedling (C) and cotyledons from (+) NaCl seedlings (D); ▸ indicates secretory canal. (E) Quantification of fluorescence from the indicated zone. Magnification: 200×.