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. Author manuscript; available in PMC: 2015 Oct 28.
Published in final edited form as: J Immunol. 2008 Feb 1;180(3):1808–1817. doi: 10.4049/jimmunol.180.3.1808

FIGURE 6.

FIGURE 6

A, Leukocyte migration across the EC matrix is inhibited by Cdk inhibitors. PBMC were allowed to adhere to the endothelial matrix for 30 min, nonadherent cells were removed, then control diluent (DMSO) or purvalanol (20 µM) was added. Cells that migrated through the Transwell filters after 4.5 h were counted. Data are presented as the mean of two independent experiments performed in triplicate ±SD and expressed as a migration index (cell number/average of control DMSO). The number of migrated cells for the control ranged from 285 to 467 in the two experiments. *, p< 0.001 compared with control. B–D, Decreased lymphocyte recruitment in Cdk4−/− mice. Wild-type (wt, WT) (n = 9), Cdk4+/−(n = 4), and Cdk4−/−(n = 5) mice were treated with bleomycin administered intratracheally. Bronchoalveolar lavage was performed 72 h later B, Cytospin demonstrating relative paucity of lymphocytes (arrows) in Cdk4−/− mice compared with wild-type mice (two mice per condition). Original magnification, X40. C, Total BALF cell count. D and E, Percent and absolute number of lymphocytes in BALF. *, p< 0.04 −/− compared with wild type.