Table 1.
Application of exogenous nitrogen dioxide (NO2) accelerated flowering and increased shoot biomass in Arabidopsis thaliana accessions C24, Columbia (Col) and Landsberg erecta (Ler)
| Accession | NO2 concentration (ppb) | Average flowering time (days) ± SD (n) | Average dry wt. of shoots (mg per plant) ± SD (n) |
|---|---|---|---|
| C24 | 0 | 42 ± 3 (20) | 25.2 ± 6.8 (4) |
| 50 | 40 ± 3 (20) * | 68.5 ± 9.3 (10) *** | |
| Col | 0 | 40 ± 3 (17) | 14.3 ± 2.5 (5) |
| 50 | 34 ± 1 (26) *** | 24.2 ± 5.5 (5) * | |
| Ler | 0 | 29.6 ± 1.5 (19) | 11.3 ± 2.6 (5) |
| 50 | 27.5 ± 2.1 (22)*** | 28.2 ± 2.3 (5) *** |
All data, excluding average flowering time of Ler, were obtained from our previous studies.17,18 Plants were harvested at 5, 4, and 4 weeks after sowing for C24, Col, and Ler, respectively (see supplementary material). The number of days after sowing when flower bolts were 1 cm in length was used as a measure of flowering time.20 Statistical significance was assessed using Dunnett's t-test for dry wt. and Student's t-test for flowering time: *, P < 0.05; ***, P < 0.001. Statistical analyses were performed using GraphPad Prism 6.0 (GraphPad Software, La Jolla, CA, USA).