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. 2014 Dec 23;15(12):1688–1699. doi: 10.4161/15384047.2014.972799

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Figure 2. — Pyoluteorin derivatives induce Mcl-1-dependent cell death through Bax and Bak in hematological cancer cell lines. Viabilities of K562/Mcl-1-IRES-Bim and K562/Bcl-2-IRES-Bim cells were measured after treatment with the indicated concentrations of KS04 (A) and KS18 (B) for 48 hours. Error bars = SD of 3 replicates. (C) Parental and ΔBak Jurkat cells were treated with the indicated concentrations of KS18 for 48 hours. (D) K562/Mcl-1-IRES-Bim and K562/Bcl-2-IRES-Bim cells were treated with 2 μM KS04 over the indicated times be analyzed through immunoblotting. K562/Mcl-1-IRES-Bim cells were treated with the indicated concentrations of KS18 (E) and KS24 (F) for 18 and 24 hours respectively and analyzed by immunoblotting.