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. 2014 Nov 3;6(6):1439–1452. doi: 10.4161/mabs.36249

Figure 3.

Figure 3.

Antigen target identification of mAb TAG-1A3 and mAb TAG-2A12. Immunoprecipitation (IP) using TAG-1A3 and commercial CD166 and, immunoblotted (IB) with (A) TAG-1A3 and (B) anti-CD 166, respectively. Results show both forward and reverse IP using the TAG-1A3 and CD166 are enriching for the same antigen bands between 64kDa and 97kDa suggesting that TAG-1A3 and CD166 are targeting at the same antigen. IP using TAG-2A12 and commercial Prdx6, IB (C) TAG-2A12, and (D) Prdx6, respectively. Results shows both forward and reverse IP using the TAG-2A12 and Prdx6 are enriching for the same antigen bands between 28kDa and 36kDa suggesting that the mAb may be targeting at the same antigen.