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. 2014 Oct 30;6(5):1243–1254. doi: 10.4161/mabs.29445

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Ryutaro Asano, Ippei Shimomura, Shota Konno, Akiko Ito, Yosuke Masakari, Ryota Orimo, Shintaro Taki, Kyoko Arai, Hiromi Ogata, Mai Okada, Shozo Furumoto, Masayoshi Onitsuka, Takeshi Omasa, Hiroki Hayashi, Yu Katayose, Michiaki Unno, Toshio Kudo, Mitsuo Umetsu, and Izumi Kumagai

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — Preparation of hEx3-scDb-Fcs with different domain orders. (A) Schematic diagrams of four types of hEx3-scDb-Fc. (B) Schematic diagrams of the expression vectors for hEx3-scDb-Fcs. (C) Gel filtration of hEx3-scDb-Fcs purified through protein A. AU, absorbance unit. (D) SDS-PAGE of each purification step in the preparation of hEx3-scDb-Fc-LH under reducing conditions (lane 1, 3) and non-reducing conditions (lane 2). Lanes 1, 2, after protein A purification; lane 3, peak fraction of gel filtration indicated by the arrow.