Table 5.

β-lactam MIC values and inhibition zone diameters found in the putative carbapenemase-producers and in carbapenem susceptible isolates selected as negative controls.

	Antimicrobial agent				Strain ID of V. cholerae isolates
		Breakpointsd			Putative carbapenemase-producers				Carbapenem susceptible controls
		S	I	R	VN-02997	VN-02825	VN-02923	VN-02808	VN-10145	VN-00301	VN-00161
MIC values (mg/L)a	Ampicillin	≤ 8	16	≥32	>32	>32	>32	>32	2	2	2
	Cefepime	≤ 8	16	≥32	0.5	0.25	0.5	0.25	0.12	≤ 0.06	≤ 0.06
	Cefoxitin	≤ 8	16	≥32	16	16	16	16	4	4	4
	Cefotaxime	≤ 1	2	≥4	0.12	≤ 0.06	≤ 0.06	≤ 0.06	≤ 0.06	≤ 0.06	≤ 0.06
	Ceftazidime	≤ 4	8	≥16	0.5	0.5	≤ 0.25	≤ 0.25	≤ 0.25	≤ 0.25	≤ 0.25
	Ertapenem	≤ 0.5	1	≥2	2	>2	>2	2	0.12	0.03	0.06
	Imipenem	≤ 1	2	≥4	16	>16	>16	16	1	0.5	1
	Temocillin	≤ 8	-	>8	4	4	4	4	4	1	1
Inhibition zone diameter (mm)b	Amoxicillin/clavulanic acid	≥18	14–17	≤ 13	14	13.5	14	15	23	28	18
	Aztreonam	≥21	18–20	≤ 17	12	15	17	16	NA	NA	29
	Cefepime	≥25	19–24	≤ 18	28	26	28	28	34	40	30
	Ceftazidime	≥21	18–20	≤ 17	28	26	28	28	NA	NA	31
	Imipenem	≥23	20–22	≤ 19	14	15	16	15	30	36	26
	Meropenem	≥23	20–22	≤ 19	20	20	20	19	34	36	28
β-lactam resistance phenotypec					(AMC)- AMP-ATM- ETP-(FOX)- IPM-(MEM)	(AMC)- AMP-ATM- ETP-(FOX)- IPM-(MEM)	(AMC)- AMP-ATM- ETP-(FOX)- IPM-(MEM)	(AMC)- AMP-ATM- ETP-(FOX)- IPM-MEM	–	–	–

MIC, minimal inhibitory concentration; S, susceptible; I, intermediate resistant; R, resistant; NA, not assessed; AMC, amoxicillin/clavulanic acid; AMP, ampicillin; ATM, aztreonam; ETP, ertapenem; FOX, cefoxitin; IPM, imipenem; MEM, meropenem.

^aMIC values obtained by broth microdilution.

^bInhibition zone diameter obtained by disk diffusion.

^cResults against non-β-lactams are not displayed; intermediate resistance is shown in brackets.

^dCriteria used for interpretation with corresponding references are given in Supplementary Table S3.