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. 2015 Apr 29;10(5):373–383. doi: 10.1080/15592294.2015.1028708

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Marcella Salzano, Marta Sanz-García, Diana M Monsalve, David S Moura, and Pedro A Lazo

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — VRK1 is required for γH2AX foci induced by IR. (A) Effect of VRK1 knockdown (si-VRK1) on γH2AX foci in A549 cells, compared or si-Control. VRK1 detection by immunoblot is at the bottom. Number of foci were analyzed by immunofluorescence confocal microscopy, counted, and represented in the graph (at the top) while the fluorescence of individual foci is shown in the graph (bottom). H2AX phosphorylation in immunofluorescence was determined using a γH2AX phospho-specific antibody. (B) Effect of VRK1 knockdown on ATM activation by IR. The phosphorylation of ATM in Ser1981 was determined in A549 cells by protein gel blot using a phospho-specific antibody. All experiments were performed at least 3 times and the number of cells counted from each experiment is the same and where analyzed by ANOVA test.