Table 1.
Selected miRNA: target pairs for validation
| Sl. No. | miRNAs | Targets (Gene IDs) | Target sequence | Free Energy | Source |
|---|---|---|---|---|---|
| 1 | miR1429_5p | MPK17–1 (LOC_Os06 g49430) | 2260-UGCAUGGAUUAAUGUAUUAU | −26.7 | PMRD |
| 2 | miR531 | MPK3 (LOC_Os03 g17700) | 571-ACAUGAUGACGGAGUACGUGGUCACCCGGUGG | −33.4 | Microinspector |
| 3 | miR531 | MPK6 (LOC_Os06 g06090) | 0-AUGGACGCCGGGGCGCAGCCGUCGGACACGGA | −36.6 | Microinspector |
| 4 | miR531 | MPK20–1 (LOC_Os01 g43910) | 31-CAGAGGCGGAUUUUUUCACGGAGUAUGGCGAU | −33.51 | Microinspector |
| 5 | miR531 | MKK10–2 (LOC_Os03 g12390) | 889-AGAAGGCCGGGCGGCGCGCGUCGGUCGGCGAG | −46.2 | Microinspector |
| 6 | miR531 | MKKK3 (LOC_Os02 g32610) | 25-CGAUGGUGCUCGGCGGCGGCGGCGGCGGCGGG | −40 | Microinspector |
| 7 | miR531 | MKKK4 (LOC_Os02 g12810) | 207-GCUCCGGUGGCGGCGGCGGCGGCGGAGCCGAG | −37.9 | Microinspector |
| 8 | miR531 | MKKK19 (LOC_Os02 g35010) | 328-GCGCGUCGGCGGCCGAGUCGGUCUCGGGCGGG | −38.8 | Microinspector |
| 9 | miR531 | MKKK23 (LOC_Os12 g40279) | 26-GCGGCUGCUGGCGGCGCUGCGGCCGUGGCUGG | −42.9 | Microinspector |
| 10 | miR531 | MKKK28 (LOC_Os03 g15570) | 702-GAGGAGGAUAGCCAUGCUCACGCCCCGGCGAG | −39.1 | Microinspector |
| 11 | miR531 | MKKK30 (LOC_Os02 g02780) | 296-GCUGGAGGAGGCGCGCGAUCCGGACCGGCGGC | −38.2 | Microinspector |
| 12 | miR531 | MKKK33 (LOC_Os02 g07790) | 184-CGGUGGACAACAGCAGCGUUGGCUCGAACGAG | −32.1 | Microinspector |
| 13 | miR531 | MKKK38 (LOC_Os06 g45300) | 179-CUUUGUUGGUGGUUCGGUUGCCAUGUCGGUGG | −31 | Microinspector |
| 14 | miR531 | MKKK45 (LOC_Os06 g43030) | 208-UGCUGGACACGGCCAUGGGGCCCUCCGGCGAG | −38.8 | Microinspector |
| 15 | miR531 | MKKK47 (LOC_Os07 g08750) | 816-CUCGCCCCGGCGGCGCGGCGGCCGGCGGCGAG | −46.1 | Microinspector |
| 16 | miR531 | MKKK52 (LOC_Os12 g06490) | 15-GCGGAGGCGGAGGAGCAGCCGCCGGACGAGGA | −42.2 | Microinspector |
| 17 | miR531 | MKKK57 (LOC_Os05 g46750) | 143-GGGGCCGGCGGGGGCGGCGCAGCUGCGGCGGG | −43.1 | Microinspector |
| 18 | miR531 | MKKK58 (LOC_Os03 g39150) | 601-AGUGCUCCUCCAAGGCGGGCACGUUCCGGUGG | −35.2 | Microinspector |
| 19 | miR531 | MKKK59 (LOC_Os12 g41260) | 35-GGUGGAGGAGAAGGCGGCGGCGGCGGCGGUGG | −36.9 | Microinspector |
| 20 | miR531 | MKKK60 (LOC_Os03 g53410) | 574-GCGUGGUGGUGGUGGAGUACCAGCACGGCGGG | −36.4 | Microinspector |
| 21 | miR531 | MKKK66 (LOC_Os10 g04010) | 82-UGUCUCUCGCGGCGGACGACGCCUCCGGCGAG | −41.4 | Microinspector |
| 22 | miR531 | MKKK67 (LOC_Os10 g04000) | 131-CGAGGGCGCCGCGAGGCAGCAGCUCCGGCGAG | −44.3 | Microinspector |
| 23 | miR531 | MKKK68 (LOC_Os12 g30570) | 409-CCGGCGGCGGCGAGCAGCGCAACACCGGCGAG | −43.2 | Microinspector |
| 24 | miR531 | MKKK69 (LOC_Os05 g46760) | 3-GCGGUGGCGGUGGCGGCGGCGGCGGCGGUGAG | −41.9 | Microinspector |
| 25 | miR531 | MKKK70 (LOC_Os01 g50410) | 113-CGCCGGCGGCGCCGCGGCGCAGCUGCGGCGAG | −45.6 | Microinspector |
| 26 | miR531 | MKKK73 (LOC_Os03 g18170) | 102-GAGGCCAAGGGCGGCGCGCCGGCGGCGGCGAU | −42.5 | Microinspector |