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. 2015 Jun 14;7(5):931–945. doi: 10.1080/19420862.2015.1055442

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Eli Lilly and Company

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Evidence for internalization and degradation of both IGF-IR and VEGF by ID2 in vitro. (A) In BxPC-3 cells, ID2 at 20 nM induces IGF-IR internalization and degradation in the presence of 100 ng/mL VEGF by immunoblotting. IR mAb and FcD2 were used as controls. (B) In A431/IGF-IR cells, ID2 at 10 nM induces both surface IGF-IR and supernatant VEGF internalization/degradation in the presence of 400 ng/mL (10 nM calculated as a dimer) VEGF by immunoblotting. IR mAb and FcD2 were used as controls. (C) Fluorescence confocal microscopy analysis on the delivery of ID2 and VEGF to the lysosome in BxPC-3 cells: (top row) the co-localization of FITC-labeled ID2 with lyso tracker; (middle and bottom row) the co-localization of FITC labeled VEGF with the lysosome is dependent on ID2 treatment. Scale bar = 20 μm.