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. 2015 Oct 28;5:15681. doi: 10.1038/srep15681

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(a) Cell proliferation with WST-1 assay of CT26 and HCT116 spheroids exposed to 10% of Conditioned Media (CM) of C. sporogenes and Reinforced Clostridium Media (RCM). t-test is a comparison with control. (*p < 0.005). (b) Area of spheroids after incubation with 10% RCM, CM and BCM of C. sporogenes at the 0, 24, 48 and 72 hour time points. The area (μm²) of the spheroids in the images was measured using ImageJ. t-test is a comparison with control (*p < 0.005, at all time points). (c) Effect of 10% CM of C. sporogenes on 3D spheroids, compared with Control, 10% RCM and 10% BCM. Scale bar represents 1000 μm.