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. 2015 Feb 19;10(4):303–318. doi: 10.1080/15592294.2015.1020000

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Figure 2. — Mycoplasma MTases translocate to the human cell nuclei and methylate the human genome. (A) substrate specificity of Mhy1 (^mCG), Mhy2 (^mCG and A^mCATGT), and Mhy3 (GAT^mC). Bisulfite DNA sequencing chromatograms of DNA substrates are shown. Stars indicate 5 mC. (B) confocal images of HT1080/Mhy1, HT1080/Mhy2, HT1080/Mhy3, HTR8/Mhy1, HTR8/Mhy3, and HTR8/mock cells. Upper left panel shows the condensed chromatin in a single HT1080/Mhy1 cell. Red, the V5 immunostaining. Blue, DAPI. (C) DNA Methylation-interference assay. HT1080/Mhy1, HT1080/Mhy2, HT1080/Mhy3 and HT1080/mock genomic DNA was digested using HpaII, MspI (a methylation-insensitive isoschizomer of HpaII), Sau3AI and MboI (a methylation-insensitive isoschizomer of Sau3AI). (D) methylation-specific PCR of a CPGI promoter region of the MMP2 gene in HT1080/Mhy1 and HT1080/mock cells. PCR products specific to methylated (M) and unmethylated (U) DNA are shown.