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Detection of mycoplasma in colorectal cancer specimens. A 270 bp PCR product specific to the mycoplasma rRNA gene was amplified from the total DNA isolated from colorectal cancer tumor (T) and matching normal (N) specimens (negative cases Cs661 and Cs694, and positive cases Cs544 and Cs583). DNA size markers and the mycoplasma-positive (HepG2 cells infected with M. hyorhinis) and negative (uninfected HepG2 cells and H2O) controls are shown.
