2095–2 amplifies in vivo platelet depletion to IdeS-generated F(ab’)2 fragments of c7E3 in rats compared with intact c7E3. (A) Treatment of rats with PBS (solid gray squares), 1 mg/kg of c7E3 (solid black circles), or 3 mg/kg of 2095–2 (solid red down triangles) did not result in platelet depletion, whereas treatment with 3 mg/kg of c7E3 (solid blue up triangles) displayed detectable platelet depletion. (B) Treatment of rats with 1 mg/kg of c7E3 F(ab’)2 fragment did not result in platelet depletion (solid gray squares), whereas the combination of 1 mg/kg of c7E3 F(ab’)2 fragment and 0.1 mg/kg of 2095–2 (solid black diamonds), 0.33 mg/kg of 2095–2 (solid red down triangles), 1 mg/kg of 2095–2 (solid blue up triangles), or 3 mg/kg of 2095–2 (solid black circles) resulted in a dose-dependent increase in platelet depletion. Data are representative of the average +/− SEM of three animals per group.