Figure 1.

Design of the IgG1/IgA2 tandem Fc fusion. Structural models of antibody formats generated for this study are shown, with IgG1- (left), an IgA2-Fc and hinge domain with IgG Fab domains (middle) and the tandem IgG1/IgA2-Fc format with an IgA2-Fc and hinge fused to an IgG-Fc (right). Structures were generated in Pymol (Schrödinger) using a human IgG1 pdb model⁴¹ and an IgA Fc structure (PDB ID: 1OW0).⁴² The DNA and encoded protein sequence of the IgG1/IgA2-Fc linkage is shown with an introduced HindIII site (boxed) highlighted.