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. 2015 Oct 28;5:15671. doi: 10.1038/srep15671

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Ct values of six 10-fold serial diluted plasmid DNAs were subject to standard curve analysis using 7500 Software v2.0.1 (Applied Biosystems) and the r² value and assay efficiency percentage were generated as 0.987 and 98.9%, respectively. The plots also show that the LOD of the assay for P. knowlesi is 1 copy number of target sequence.