Table 1. Comparison of the loadings from canonical variate analysis (CVA) of all 3 oxygen conditions.
| Metabolite | HCT 116: loading common between WT and DN | HEPA-1: loading common between WT and C4 |
|---|---|---|
| 4-aminobutyrate | normoxia | |
| 4-hydroxyproline | normoxia | |
| Allose/Mannose/Galactose /Glucose (1) | * | * |
| Allose/Mannose/Galactose /Glucose (2) | * | * |
| Allose/Mannose/Galactose /Glucose (3) | normoxia | |
| 2-hydroxyglutarate | low oxygen | |
| 2-oxoglutarate | normoxia* | normoxia* |
| Aspartate (1) | normoxia | |
| Aspartate (2) | normoxia* | normoxia* |
| Beta-alanine | normoxia | |
| Citrate | normoxia* | normoxia* |
| Creatinine | low oxygen | |
| Cysteine | normoxia | |
| Erythronate /Threonate | low oxygen* | low oxygen* |
| Fructose (1) | * | * |
| Fructose (2) | normoxia | |
| Fructose / Sorbose (1) | low oxygen | |
| Fructose / Sorbose (2) | low oxygen* | low oxygen* |
| Glutamate | normoxia | low oxygen |
| Glutamine | normoxia* | normoxia* |
| Glycerol | low oxygen* | low oxygen* |
| Glycerol-3-phosphate | low oxygen | |
| Glycine | low oxygen | |
| Hexadecanoic acid | * | * |
| Hypotaurine | low oxygen* | low oxygen* |
| Lactate | low oxygen* | low oxygen* |
| Leucine | N/A | |
| Lysine | low oxygen | |
| Malate | normoxia | |
| Malonate | low oxygen* | low oxygen* |
| Methionine | low oxygen* | low oxygen* |
| Norleucine | low oxygen* | low oxygen* |
| Phosphate | low oxygen* | low oxygen* |
| Phosphocreatinine | N/A | |
| Putrescine | normoxia* | normoxia* |
| Pyroglutamate | normoxia | |
| Pyruvate | normoxia | |
| Scyllo-/Myo-inositol | * | * |
| Octadecenoic acid | * | * |
| Threonine | low oxygen* | low oxygen* |
| Tyramine/Tyrosine | low oxygen | |
| Tyrosine | normoxia* | normoxia* |
| xylitol/Ribitol | low oxygen | N/A |
CVA was performed for HCT 116 wild type (WT) samples, HCT 116 dominant negative (DN) samples HEPA-1 WT samples and HEPA-1 C4 samples (Fig. 3a–d). Metabolites labelled ‘normoxia’ were identified in the loadings as being features of normoxia that were common between WT and HIF-1 deficient cells, while metabolites labelled ‘low oxygen’ were identified in the loadings as being features of low oxygen that were common between WT and hypoxia inducible factor 1 (HIF-1) deficient cells. Un-labelled metabolites represent HIF-1 mediated responses to oxygen since the loadings were not common between WT and HIF-1 deficient cells. Metabolites marked with an asterisk (*) indicate cell line similarities such that the same behaviour was observed in both HCT 116 and HEPA-1cell lines.