Figure 3.

Overexpression of NDRG2 causes downregulation of PSA but not affects AR quantity and activity in LNCaP and 22Rv1 cells. (A) Expression pattern of NDRG2 and the designated proteins upon NDRG2 overexpression. Cells infected with lentivirus encoding NDRG2 and LacZ were lysed, and Western blots were performed using the designated antibodies. α-Tubulin protein levels were used as a loading control. Representative blots are shown, and the relative expression levels of the different proteins normalized to α-Tubulin were derived from 4 independent experiments (n = 4). (B) Immunofluorescence detection of AR subcellular localization pattern with anti-AR antibody in Lenti-NDRG2-infected and control cells (Scale bar: 20 μm). (C) ELISA analysis of PSA secretion in Lenti-NDRG2-infected and control cells. The histograms were drawn based on the average values (n = 3). In A and C, data indicate the mean ± SD *: P < 0.05; **: P < 0.01; ***: P < 0.001.