Figure 6. The involvement of NO in fructose, glucose, sucrose and glycerol-mediated innate immunity against Pst DC3000 in Arabidopsis.

For the assay, 26-day-old soil-grown WT (Col-0) and NO deficient mutants (noa1 and nia1nia2) were watered with nutrient solution containing mock, 5 mM fructose, 5 mM glucose, 5 mM sucrose and 5 mM glycerol from below in pots with plants for 2 days, and then 28-day-old soil-grown WT (Col-0) and NO deficient mutants (noa1 and nia1nia2) were infected with bacterial suspension (OD₆₀₀ = 0.002) containg 10 mM MgCl₂ and 0.05% silwet L-77. At 0, 2 and 4 days post infection (dpi), the bacterial populations in the leave discs were determined. The results shown are the means ± SDs (n = 6).