Figure 7. The effects of exogenous treatments of fructose, glucose, sucrose and glycerol on endogenous levels of melatonin (a) and NO (b) (c) in Arabidopsis.

For the assay, 26-day-old soil-grown WT (Col-0) Arabidopsis plants were watered with nutrient solution containing mock, 5 mM fructose, 5 mM glucose, 5 mM sucrose and 5 mM glycerol from below in pots with plants for 0, 3, 6, 12 and 24 hrs, respectively, and plant leaves were used for the determination. Endogenous NO level in the leaves were assayed using the hemoglobin assay (b) and the DAF-FM DA assay (c). The results shown are the means ± SDs (n = 3). The sterisk (*) indicates significant difference in comparison to 0 hr of treatment at p < 0.05.