Table 1.
Overview of PsAOMT and PtAOMT activities with various substrates under optimized assay conditions
| Substrate | PsAOMT | PtAOMT | ||||
|---|---|---|---|---|---|---|
| K m (μM) | V max (nM–1) | K cat× 10–3 (s–1) | K cat /K m (M–1s–1) | Specific activity (pkat mg–1) | Specific activity (pkat mg–1) | |
| Pelargonidin 3-O-glucoside | – | – | – | – | – | – |
| Cyanidin 3,5-di-O-glucoside | 1.06 (0.01) | 17.88 (0.25) | 127.70 (1.05) | 120886 (563) | 1788 (15) | 29.60 (3.47) |
| Cyanidin 3-O-glucoside | 1.76 (0.02) | 11.57 (0.05) | 161.99 (0.73) | 91829 (421) | 2314 (11) | 37.98 (1.05) |
| Delphinidin 3-O-glucoside | 4.11 (0.35) | 17.05 (0.27) | 94.72 (1.49) | 23293 (1538) | 1364 (21) | 21.50 (0.10) |
| Quercetin 3-O-rutinoside | 12.32 (0.32) | 27.44 (1.63) | 192.11 (4.41) | 15599 (110) | 2744 (135) | 30.20 (0.24) |
| Luteolin 7-O-glucoside | 146.26 (10.07) | 31.61 (1.64) | 0.22 (0.00) | 1486 (145) | 2017 (109) | 2.24 (0.18) |
| Kaempferol 3-O-glucoside | – | – | – | – | – | – |
| Cyanidin | – | – | – | – | 0.40 (0.05) | – |
| Delphinidin | – | – | – | – | – | – |
| Quercetin | 4.33 (0.07) | 1.77 (0.02) | 9.83 (0.12) | 2271 (24) | 142 (2) | 17.58 (0.57) |
| Luteolin | – | – | – | – | 0.043 (0.002) | 2.08 (0.27) |
| Kaempferol | – | – | – | – | – | – |
| Apigenin | – | – | – | – | – | – |
| Naringenin | – | – | – | – | – | – |
| Epicatechin | – | – | – | – | – | – |
| Caffeic acid | – | – | – | – | – | – |