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. 2015 Jul 28;66(21):6579–6589. doi: 10.1093/jxb/erv366

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Histological analysis of apple skin tissues in a selected subset of the population. Light microscopy analysis of skin samples stained with Sudan IV are shown, together with photographs of whole apples. Sudan IV stains the cuticular lipids a pink/orange colour. (A, B) Parental lines ‘Golden Delicious’ (A) and ‘Braeburn’ (B), show regular cuticles. (C–E) Selected progeny with regular skin are shown: (C) G×B 3; (D) G×B 6; (E) G×B 11. (F–H) Selected progeny with compromised cuticles are shown: (F) G×B 30; (G) G×B 59; (H) G×B 90. Thinner, cracked cuticles are clearly visible in these lines, whereas whole apple photographs show the presence of russet on the surface of the fruit.