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. 2015 Jul 27;66(21):6591–6603. doi: 10.1093/jxb/erv367

Fig. 5.

Fig. 5.

Effect of knock-down of the wheat (Triticum aestivum) AGC kinase TaAGC1 gene on the resistance response of the resistant wheat strain CI12633 to Rhizoctonia cerealis. (A) RT-PCR analysis of the transcription levels of the barley stripe mosaic virus (BSMV) CP gene and typical symptoms of BSMV in the fourth leaves of wheat plants infected by BSMV:GFP or BSMV:TaAGC1. (B) qRT-PCR analysis of the relative transcript levels of TaAGC1 in the wheat plants infected by BSMV:GFP or BSMV:TaAGC1 at 14 dpi with R. cerealis. The relative transcript level of TaAGC1 in the wheat CI12633 plants infected by BSMV:TaAGC1 is relative to that in BSMV:GFP-infected wheat CI12633 plants. Three biological replicates per line were averaged and statistically treated (t-test; ** P<0.01). Bars indicate standard error of the mean. (C) Sharp eyespot symptoms of the BSMV:GFP- and BSMV:TaAGC1-inoculated CI12633 plants at 14 and 40 dpi with R. cerealis. Similar results were obtained from three independent replicates. (This figure is available in colour at JXB online.)