Fig. 7.

Wheat (Triticum aestivum) AGC kinase TaAGC1 modulates reactive oxygen species (ROS) levels and the expression of ROS-related genes. (A) Analysis of hydrogen peroxide (H₂O₂) and superoxide anion (O₂ ⁻) levels in wheat. Leaves were harvested from TaAGC1-overexpressors (PK13, PK35 and PK37) and WT wheat plants at 0h and 4 dpi with Rhizoctonia cerealis, and were then stained with 3,3ʹ-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) to detect H₂O₂ and O₂ ⁻, respectively. Similar results were obtained from three independent replicates. (B) Transcriptional analysis of genes encoding ROS-scavenging enzymes (POX2, CAT1 and SOD3) and the ROS-producing enzyme NOX in wheat. The tested wheat samples include TaAGC1-overexpressors (PK13, PK35 and PK37), untransformed wheat Yangmai 20 (WT) and TaAGC1-knock-down plants (BSMV:TaAGC1-1, -2 and -3) and BSMV-GFP-infected controls at 7 dpi with Rhizoctonia cerealis. The reported transcript levels of the tested gene in the transgenic plants are relative to those in the WT plants. Statistically significant differences of TaAGC1-overexpression or TaAGC1-knock-down wheat plants were compared with the WT or the control, and based on three technical replications (t-test; *P<0.05, **P<0.01). Bars indicate standard error of the mean. (This figure is available in colour at JXB online.)