Table 1.
Rhizoctonia cerealis responses in transgenic wheat (Triticum aestivum) lines overexpressing the AGC kinase gene TaAGC1 and wild-type (WT) wheat
| Lines | Infection type | Disease index | ||||
|---|---|---|---|---|---|---|
| T 1 | T 2 | T 3 | T 1 | T 2 | T 3 | |
| PK13 | 1.38** | 2.17** | 1.63** | 27.50** | 43.43** | 32.66** |
| PK35 | 1.63** | 1.86** | 1.62** | 32.50** | 37.14** | 32.36** |
| PK37 | 1.62** | 1.98** | 1.73** | 32.42** | 39.67** | 34.61** |
| WT (Yangmai 20) | 2.28 | 3.84 | 2.72 | 45.56 | 76.89 | 54.38 |
** Significant difference between each transgenic line and untransformed (WT) wheat Yangmai 20 at P<0.01 (t-test). Infection types (ITs) were categorized from 0 to 5: 0, no lesion; 1, the lesion appeared on the sheaths rather than stems; 2, the width of the lesion is <50% of the infected stem perimeter; 3, the width of the lesion is >50 and <75% of the infected stem perimeter; 4, the width of the disease lesion is >75% of the infected stem perimeter; 5, white spike or dead plant. The infection types were the average ITs of 30 plants for each line of the TaAGC1 transgenic wheat in T1−T3 generations and WT wheat Yangmai 20 at 50 dpi with R. cerealis. Disease index={(0×X0+1×Xl+2×X2+3×X3+ 4×X4+5×X5)/[(X0+X1+X2+X3+X4+X5)×5]} ×100, where X0−X5 indicated plants with IT: 0−5. The representative symptoms of different ITs are shown in Supplementary Fig. S2.