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. 2015 Aug 17;66(21):6835–6847. doi: 10.1093/jxb/erv389

Fig. 4.

Fig. 4.

Tuber production (A) for independent Désirée transgenic overexpression lines for PTB1 (#9 lines) and PTB6 (#10 lines). Four to five replicates from select overexpression lines were grown in 13cm pots. Tuber yields (g FW per plant) were assessed at 45 d. Plants were grown under long-day conditions (16h light, 8h dark) with a fluence rate of 300 mmol m–2 s–1 at 24 °C during a 12h day cycle and 18 °C for the remaining time. Relative RNA accumulation of StBEL5 (B), GA2 oxidase1 (C), and StPIN1 (D) in stolons of PTB overexpression lines after 30 d in soil. RNA was calculated using qRT–PCR and is shown in relation to wild-type (WT) levels. Des, wild-type Désirée control; R1, transgenic Désirée GFP control; OEP1G, overexpression line of StPTB1; OEP6G, overexpression line of StPTB6. Standard deviations of the means of three biological replicates are shown, with one, two, and three asterisks indicating significant differences (P<0.05, P<0.01, P<0.001, respectively) using a Student’s t-test.