Fig. 8.

Expression levels of COMT transcript and ASMT enzyme activity. (A) RT-PCR analysis of COMT mRNA in wild-type and transgenic lines. (B) ASMT enzyme activity in the shoot of wild-type and transgenic lines. (C) ASMT enzyme activity in the root of wild-type and transgenic lines. Seven-day-old seedlings grown in a half strength MS medium were employed. The numbers in parentheses indicate the number of PCR cycles used. Asterisks (*) indicate significant differences from the wild-type (P<0.05). WT, wild-type; OX lines, COMT-overexpressed transgenic lines (T2); RNAi lines, COMT-suppressed RNAi transgenic lines (T2).