Figure 5. Correlation spectra of VpuFull in DMPC proteoliposomes.

A. 13C/13C correlation spectrum obtained with 50 ms PDSD mixing. B. 13C/13C TOBSY spectrum; the J-coupling based 13C/13C correlation spectrum indicates the mobile residues of VpuFull. C. 15N/13C HETCOR spectrum, where the 15N/13C transfer is obtained from SPECIFIC-CP. All of the experiments show that single-site resolution is achievable when VpuFull is incorporated in DMPC proteoliposomes at 25 °C.