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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Sep 8;1848(11 0 0):3007–3018. doi: 10.1016/j.bbamem.2015.09.008

Figure 6. Solid-state NMR measurements for structure calculation of VpuFull in DMPC proteoliposomes.

Figure 6

A. Plot of 1H-15N dipolar couplings versus residue numbers, indicating the helical regions in VpuFull. B. Overlap of 13C/13C TOBSY spectrum of L41C-VpuFull with MTSL (red) and its reduced diamagnetic form (black) at the alanine region. C. Overlap of 13C/13C TOBSY spectrum of L41C-VpuFull with MTSL (red) and its reduced diamagnetic form (black) at the serine region. D. Overlap of 13C/13C TOBSY spectrum of VpuFull with (blue) and without (black) MnEDTA present at the alanine region. E. Overlap of 13C/13C TOBSY spectrum of VpuFull with (blue) and without (black) MnEDTA present at the serine region. F. Plot of residues intensity change caused by L41C-MTSL. G. Plot of residues intensity change caused upon addition of MnEDTA solution.