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. 2015 Oct 28;5:15906. doi: 10.1038/srep15906

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Copyright © 2015, Macmillan Publishers Limited

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(A–C) Dual-luciferase reporter assay for validation of miR-200s target sites in the 3′ UTR of GH, GHRb, GHRa and IGF2a. Conserved miR-200–binding sites in GH and GHRb 3′ UTR are indicated and mutated separately. Luciferase assays were performed in triplicate and are representative of 2–3 independent experiments. (D) Levels of miR-141 and miR-429a at 24 hpf embryos injected with control or miRNA inhibitors. (E) Expression of GH/IGF axis genes in embryos following injection of miRNA inhibitors. (A–E) Error bars indicate mean ± SD, n = 3. Student’s t-test was used for statistical analysis (*p < 0.05).