Table 2.
Lack of HIV-1 replication in PHA-activated CD4+T lymphocytes challenged with different exosome preparations
| Days post-challenge input | CAp24 pg/ml | % of HIV-1+ cells | ||||
|---|---|---|---|---|---|---|
| 2 | 5 | 7 | 2 | 5 | 7 | |
| Exo U937 | <15 | <15 | <15 | <1 | <1 | <1 |
| Exo U937 Δnef HIV-1 | 31 | <15 | <15 | <1 | <1 | <1 |
| Exo U937 Δnef
HIV-1 + wtNef |
26 | <15 | <15 | <1 | <1 | <1 |
| Exo U937 Δnef
HIV-1 + NefG2A |
44 | <15 | <15 | <1 | <1 | <1 |
| Exo CD4+ T lymphocytes | <15 | <15 | <15 | <1 | <1 | <1 |
| Exo CD4+ T lymphocytes + HIV-1 | <15 | <15 | <15 | <1 | <1 | <1 |
| Exo MDMs | <15 | <15 | <15 | <1 | <1 | <1 |
| Exo MDMs + HIV-1 | <15 | <15 | <15 | <1 | <1 | <1 |
| Exo Hut-78 | <15 | <15 | <15 | <1 | <1 | <1 |
| Exo Hut-78/F12 | 89 | <15 | <15 | <1 | <1 | <1 |
| HIV-1, 50 pg | <15 | 9980 | 2400 | <1 | 32.2 | 7.9 |
A total of 5 × 104 CD4+ T lymphocytes were spinoculated with 120 μU of the exosome preparations indicated in the left column. As a control, the same number of cells was challenged with 50 pg CAp24 equivalents of the T-tropic NL4-3 HIV-1 strain. Cell cultures were washed 24 h later, and viral replication was monitored at the indicated days post-infection by measuring CAp24 levels in supernatants as well as scoring HIV-1 expressing cells by CAp24 FACS analysis. Shown are mean values obtained by challenging cells from two healthy donors with duplicate conditions