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. 2015 Oct 27;10(10):e0141378. doi: 10.1371/journal.pone.0141378

Fig 2. Analysis of the effects of LF on the activation of ERK1/2 and Ras.

Fig 2

(A) Activation of ERK1/2 (Thr202/Tyr204) after treatment of adipocytes with LF. Phosphorylated ERK1/2 was detected in the presence or absence (0 min) of 1 mg/ml of LF. Phosphorylation levels normalized to protein expression levels of ERK1/2 are shown. (B) Ras activation through c-Raf in adipocytes treated with LF. Activated Ras captured from cell lysates using a pull-down assay kit (see Experimental Procedures) before (0 min) and after treatment with 1mg/ml of LF. Activated Ras eluted from the beads was detected using western blot analysis. Intensity levels normalized to the total protein determined by BCA. The statistical significance of the data at each sampling time compared with the 0-min sample was evaluated using Dunnett’s multiple comparison test, and the data represent the mean ± SD values of triplicate determinations of one of three identical experiments. *p < 0.05, **p < 0.01, ***p < 0.001. ERK, extracellular signal-regulated kinase; LF, lactoferrin; SD, standard deviation.