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. 2015 Oct 27;10(10):e0141378. doi: 10.1371/journal.pone.0141378

Fig 4. The influence of PKA activity inhibition on the downstream factor CREB.

Fig 4

Phosphorylation level of CREB-Ser133 was detected 15 min after the treatment with 1 mg/ml of LF with or without H-89, a selective PKA inhibitor. Adipocytes were pre-incubated in H-89 starting at 30 min before the addition of LF. Phosphorylation level was normalized to the CREB protein expression level. The statistical significance of the differences in the data for LF treated vs. untreated samples was evaluated using the Student t test. **p < 0.01, n.s.; no significant difference. The data represent the mean ± SD of triplicate determinations of one of the three identical experiments. LF, lactoferrin; PKA, protein kinase A: CREB, cAMP response element binding protein: HSL, hormone sensitive lipase: SD, standard deviation.