Fig 7. The influence of a βAR-blocker on the activities of lipolysis inducers.

(A) Activation of lipolysis in adipocytes by ISO with or without a βAR inhibitor. The amount of glycerol in the medium was analyzed 24 h after adding 0.1 mM of ISO with or without atenolol (β₁AR blocker) to quantitate lipolysis. The statistical significance of the differences in the data for LF treated vs. untreated cells was evaluated using the Student’s t test. ***p < 0.001, n.s.; no significant difference. The data represent the mean ± SD values of triplicate determinations of one of the three identical experiments. (B) Activation of lipolysis by LF with or without βAR inhibitor. The amount of glycerol in the medium was analyzed 24 h after adding 1 mg/ml of LF with or without atenolol (β₁AR blocker) to quantify lipolysis. The statistical significance of the data of LF treated and untreated cells were evaluated using Student’s t test. *p < 0.05, ^† p < 0.1, n.s.; no significant difference. The data represent the mean ± SD values of triplicate determinations of one of the three identical experiments. LF, lactoferrin; SD, standard deviation.